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The Vitamin D (25-Hydroxy Vitamin D) test measures the level of 25-hydroxy vitamin D [25(OH)D] in the blood, the primary storage form of vitamin D and the most reliable indicator of the body’s vitamin D status. Vitamin D is essential for calcium absorption, healthy bones and teeth, muscle function, and immune system support. It is produced in the skin through sunlight exposure and is also obtained from dietary sources and supplements. This test helps identify vitamin D deficiency or excess and evaluate disorders affecting bone and mineral metabolism.
The Vitamin D (NHC) test is recommended for individuals with bone pain, muscle weakness, frequent fractures, osteoporosis, fatigue, recurrent infections, limited sun exposure, or suspected vitamin D deficiency. It is commonly used to diagnose rickets in children, osteomalacia in adults, osteoporosis, and calcium metabolism disorders, as well as to monitor vitamin D supplementation and treatment response. Results are often interpreted together with calcium, phosphorus, parathyroid hormone (PTH), and kidney function tests for a comprehensive evaluation. At Nidan Pathology Lab, we provide NABL-accredited testing, FREE home sample collection, advanced laboratory technology, and accurate, timely reports to support the diagnosis and management of vitamin D-related disorders.
Vitamin D (NHC) Test-
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