TB GOLD INTERFERON GAMMA RELEASE ASSAY (IGRA) (NHC)

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The TB GOLD Interferon Gamma Release Assay (IGRA) (NHC) is an advanced blood test used to detect latent tuberculosis infection (LTBI) by measuring the body’s cell-mediated immune response to specific Mycobacterium tuberculosis antigens. Unlike the traditional Tuberculin Skin Test (TST), the IGRA test is not usually affected by prior BCG (Bacillus Calmette-Guérin) vaccination, making it a highly specific method for identifying latent TB infection. A positive result suggests that infection with Mycobacterium tuberculosis is likely, although additional clinical evaluation and diagnostic tests are required to determine whether the infection is latent or active.

This test is commonly recommended for individuals who have been in close contact with TB patients, healthcare workers, people with weakened immune systems, and those at increased risk of tuberculosis. The test requires a blood sample collected in special QuantiFERON-TB Gold tubes or a heparinized blood tube, following specific collection and transport guidelines. No special patient preparation is required. Early detection of latent TB allows healthcare providers to consider preventive treatment, reducing the risk of progression to active tuberculosis and helping control the spread of the disease.

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The TB GOLD Interferon Gamma Release Assay (IGRA) (NHC) Test-

What is the TB GOLD Interferon Gamma Release Assay (IGRA) test?

The TB GOLD IGRA test is a blood test that detects latent tuberculosis infection (LTBI) by measuring the immune system’s response to specific Mycobacterium tuberculosis antigens.

Why is this test performed?

The test is performed to identify latent TB infection, assess individuals at high risk of tuberculosis, and help determine the need for preventive treatment before the infection progresses to active TB.

Does a positive result mean I have active tuberculosis?

No. A positive result indicates that infection with Mycobacterium tuberculosis is likely, but additional medical evaluation, such as a chest X-ray and other diagnostic tests, is required to determine whether the infection is latent or active.

Is the TB GOLD IGRA test affected by BCG vaccination?

No. Unlike the Tuberculin Skin Test (TST), the TB GOLD IGRA test is generally not affected by prior BCG vaccination, making it more specific for detecting TB infection.

Is any special preparation required before the test?

No. No special preparation or fasting is required. A blood sample is collected according to laboratory guidelines.

Why is the TB GOLD IGRA test important?

The test helps detect latent tuberculosis early, supports preventive treatment, reduces the risk of developing active TB, and contributes to better tuberculosis control and patient care.

The TB GOLD Interferon Gamma Release Assay (IGRA) (NHC)

Latent tuberculosis infection (LTBI) is a non-communicable asymptomatic condition that persists for many years and may progress to tuberculous disease. The main aim of diagnosing LTBI is to consider medical treatment for preventing the disease. This test is a measure of cell mediated immune response to antigens simulating the mycobacterial proteins. A positive result indicates that mycobacterium tuberculosis infection is likely but further medical & diagnostic evaluation is necessary. This test is usually negative in individuals vaccinated with Mycobacterium bovis BCG.

Purpose of the Test:

Detect latent tuberculosis infection (LTBI).
Measure the immune response to Mycobacterium tuberculosis antigens.
Identify individuals at risk of developing active tuberculosis.
Support early diagnosis and preventive treatment decisions.
Screen high-risk individuals, including healthcare workers and close TB contacts.

Symptoms:

Close contact with a person diagnosed with TB.
Weakened immune system (e.g., HIV infection or immunosuppressive therapy).
Persistent cough, fever, or weight loss if active TB is suspected.
History of exposure to tuberculosis.
Increased risk of TB due to occupational or medical factors.

Importance:

Detects latent tuberculosis before it progresses to active disease.
Not usually affected by prior BCG vaccination, making it highly specific.
Helps doctors identify individuals who may benefit from preventive treatment.
Supports early intervention and reduces the risk of TB transmission.
Improves tuberculosis control through timely diagnosis and management.

Instructions:

Collect 1 mL whole blood each in a set of special QuantiFERON – TB Gold tubes Shake the tubes vigorously at least 10 times to ensure thorough mixing. DO NOT CENTRIFUGE. Ship at room temperature to reach lab within 16 hours OR Incubate the tubes immediately at 37°C for 16-24 hours and centrifuge. Ship refrigerated OR 4 mL (3 mL min.) whole blood from 1 Green top (Sodium/Lithium heparin) tube. Ship refrigerated. DO NOT FREEZE.

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