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The Vitamin D, 25-Hydroxy test measures the level of 25-hydroxy vitamin D [25(OH)D], the primary storage and circulating form of vitamin D in the body. It is the most reliable test for assessing vitamin D status and helps evaluate bone health, calcium absorption, and overall mineral metabolism. This test measures both Vitamin D2 and Vitamin D3 components, providing a comprehensive assessment of vitamin D levels. Vitamin D deficiency is common, particularly in older adults, and may lead to osteoporosis, secondary hyperparathyroidism, rickets in children, and osteomalacia in adults, while excessive vitamin D levels may indicate hypervitaminosis D.
The Vitamin D, 25-Hydroxy test is recommended for individuals with bone pain, muscle weakness, recurrent fractures, osteoporosis, limited sun exposure, malabsorption disorders, or suspected vitamin D deficiency. It is also used to investigate the causes of rickets and osteomalacia, monitor vitamin D replacement therapy, and assess overall bone and metabolic health. At Nidan Pathology Lab, we provide NABL-accredited testing, FREE home sample collection, advanced laboratory technology, and accurate, timely reports to support the diagnosis and effective management of vitamin D-related disorders.
Vitamin D, 25 - Hydroxy (NHC) Test-
25-Hydroxy vitamin D represents the main body reservoir and transport form. Mild to moderate deficiency is associated with Osteoporosis / Secondary Hyperparathyroidism while severe deficiency causes Rickets in children and Osteomalacia in adults. Prevalence of Vitamin D deficiency is approximately >50% specially in the elderly. This assay is useful for diagnosis of vitamin D deficiency and Hypervitaminosis D. It is also used for differential diagnosis of causes of Rickets & Osteomalacia and for monitoring Vitamin D replacement therapy.
This assay measures both D2 and D3 components.
No special preparation required
3 mL (1.5 mL min.) serum from 1 SST.
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